Publicação Destaque
Accessing the biocompatibility of layered double hydroxide by intramuscular implantation: histological and microcirculation evaluation
DOI: 10.1038/srep30547
Vanessa Roberta Rodrigues Cunha, Rodrigo Barbosa de Souza, Ana Maria Cristina Rebello Pinto da Fonseca Martins,
Ivan Hong Jun Koh & Vera Regina Leopoldo Constantino
Biocompatibility of layered double hydroxides (LDHs), also known as hydrotalcite-like materials or
double metal hydroxides, was investigated by in vivo assays via intramuscular tablets implantation in
rat abdominal wall. The tablets were composed by chloride ions intercalated into LDH of magnesium/
aluminum (Mg2Al-Cl) and zinc/aluminum (Zn2Al-Cl). The antigenicity and tissue integration capacity
of LDHs were assessed histologically after 7 and 28 days post-implantation. No fibrous capsule nearby
the LDH was noticed for both materials as well any sign of inflammatory reactions. Sidestream Dark
Field imaging, used to monitor in real time the microcirculation in tissues, revealed overall integrity of
the microcirculatory network neighboring the tablets, with no blood flow obstruction, bleeding and/
or increasing of leukocyte endothelial adhesion. After 28 days Mg2Al-Cl promoted multiple collagen
invaginations (mostly collagen type-I) among its fragments while Zn2Al-Cl induced predominantly
collagen type–III. This work supports previous results in the literature about LDHs compatibility with
living matter, endorsing them as functional materials for biomedical applications.